Webinar Series

2017

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Special Webinar on Fish Health

DateFriday June 16, 2017
Time: 1130 EST

“Factors Influencing zebrafish egg size”- Dante D’India, Harvard Medical School

“Heavy metal contaminated diet affects survival, health, and development of larval zebrafish” Marc Tye, University of Minnesota

“Fish Health and Biosecurity” Dr. Charles Innis, New England Aquarium


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Zebrafish Health Screening (and quarantine); why and how should we screen for pathogens?

Presenter: Joanna Cambray-Young, The University of Sheffield
Date: Wednesday, January 11, 2017
Time: 10am CST

In rodent research facilities, health screening has been conducted routinely for many decades. If pathogens are found to be present, it may be necessary to eliminate pathogens that are likely to be detrimental to animal welfare or the scientific outcomes. With the recent availability of PCR tests for zebrafish pathogens, health screening is now becoming more commonplace in zebrafish facilities across the world. How the screen is implemented and which samples are taken will drastically affect the interpretation and usefulness of the test results. During this seminar I will give my views on why we should health screen our fish and how we might achieve this. A natural extension of this is the role of quarantine in maintaining the biosecurity of zebrafish facilities


2016

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Implementaion of the ARRIVE (Animal Resarch: Reporting of In Vivo Experiments) Guidelines for Zebrafish Research

Presenter: Dr. Claire Allen, University of Sheffield
Date: May 18, 2016
Time: 12:00 PM EST

The ARRIVE guidelines were developed by the National Centre for the 3Rs of Animal Research in the U.K.  These are international recommendations to improve the design, analysis and reporting of research using animals. They aim to maximize information published and minimize unnecessary suffering.  These guidelines are extensively endorsed by scientific journals, major funding bodies and requirements of the guidelines as well as discuss how zebrafish facilities can implement them and assist in their fulfillment for publication.


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Danio musculus: Taking Principles Learned From Mouse Enrichment and Applying Those to Improve the Lives of Research Zebrafish

Presenter: Michael Esmail, VMD DLAM, Tufts University
Date: August 23, 2016
Time: 12:00 PM CST

What is enrichment?  Do zebrafish need it?  How can we use the knowledge we have with mice and apply it to zebrafish?  These questions and more will be discussed during the next ZHA Webinar presentation.



2015

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Optimizing Strategies for Detecting and Mitigating Disease in Zebrafish Research Systems

Presenter: Dr. Dave Marancik, Aquatic Veterinarian, Fish Vet Group
Date:  December 3, 2015
Time:  2:00 PM EST

This presentation outlines our recent studies that strive to understand the best approaches for detecting and monitoring disease in zebrafish research systems.  This includes objectively comparing methodologies used to screen zebrafish for disease, including assay-type, sample number, and fish location.  We also discuss results from a challenge model being used to characterize pathogenicity of isolated bacteria.  These efforts provide guidance for an evidence-based approach to disease management, biosecurity and fish health.

Please RSVP to admin@zhaonline.org by November 30.
Please Note: You must be a paid member of ZHA to participate in this webinar. Click Here membership details. If you have any questions about your membership, please email admin@zhaonline.org


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Keeping Pathogens out of Your Zebrafish Facility

Presenter:  Dr. Christopher Whipps, Associate Professor and Director of Center for Applied Microbiology, SUNY-ESF, Syracuse NY
Date:  July 22, 2015
Time: 2:00 PM EST

An important aspect of minimizing disease in zebrafish colonies is limiting opportunities where new pathogens can be introduced. It is recommended that when new fish are introduced to a facility, it is only through the introduction of bleached embryos or eggs. Yet, the effectiveness of these methods have not been thoroughly evaluated, and there may be other methods that work just as well or better. This webinar covers some of the approaches for limiting introduction of pathogens and suggestions for successful control.

Please send RSVP to admin@zhaonline.org by July 17, 2015 if interested in participating.  This presentation is only available to current ZHA members.  Click on the membership tab above for details on how to join or renew your membership.


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Preparing Your Facility for IACUC Inspections

Presenter:  Dr. George Sanders, Lecturer & Aquatic Animal Program Director, University of  Washington School of Medicine
Date:  March 31, 2015
Time: 3:00 PM EST
Open to all interested parties, membership not required.
Please send RSVP to admin@zhaonline.org if interested in participating.

Due to the rapid growth of the zebrafish model over the past years, Institutional Animal Care and Use Committees (IACUC) have been increasingly required to approve and inspect these research animal housing and procedure areas more so than before. Without individuals knowledgeable about the required care of this model on the IACUC and present during these site visit, it can be difficult to have a reliably fruitful inspection result. In this regard, preparing for and conducting appropriate IACUC site visits and inspections is an institutional team effort. This webinar series will discuss the planning and preparations necessary in order to have a successful IACUC inspection of your zebrafish facility.



2014

ZEBRAFISH PATHOGENS: IMPACT ON RESEARCH

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Presenter: Marcus Crim of IDEXX RADIL
Date: January 22, 2014 @ 1pm EST
Status: Completed
Number of attendees:

The use of laboratory zebrafish has expanded dramatically from developmental genetics into diverse new areas of biomedical research.  Many of these fields, including aging, behavior, cancer, growth, immunity, infection, metabolism, and toxicology use adult zebrafish in addition to embryos and larvae.  Several studies now document the confounding effects of subclinical disease in laboratory zebrafish, and it is well established in other animal models that unrecognized clinical or subclinical infections can result in invalid or confounded experimental results. This presentation will provide an overview of the potential impact of undetected infections on biomedical research using embryos and adult zebrafish, including examples from zebrafish as well as other species, and a discussion of the utility of sentinel health monitoring in protecting the validity of experimental data.


BRINGING CRYOPRESERVATION AND FERTILIZATION TO THE NEXT LEVEL

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Presenter: Carrie Carmichael and Chapell Miller of Cryogenetics
Date: April 24, 2014 @ 1pm EST
Status: Completed
Number of attendees registered: 48

Cryogenetics is the world leader in fish reproduction technologies. It has developed successful protocols for cryopreservation of milt (sperm) from more than 10 fish species, and provides products and services worldwide. Early in 2014, Cryogenetics, Inc. opened a new laboratory in Greater Boston, MA.  This specialized laboratory is equipped to receive and maintain live zebrafish from clients.  Cryogenetics has also developed a new cryopreservation protocol and designed a small volume container for zebrafish sperm.  Tests have shown an average of 85% post thaw fertilization rate.  Cryopreservation is a valuable tool for gene back up and preservation and is an important aspect of fish colony management.


THE EVOLUTION OF AQUACULTURE RESEARCH INFRASTRUCTURE AT BOSTON CHILDREN’S HOSPITAL

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Presenter: Christian Lawrence of Boston Children’s Hospital
Date: September 17, 2014
Time: TBD

The tremendous explosion in growth in the zebrafish model system over the past two decades has been accompanied by a concomitant increase in the creation of dedicated, physical infrastructure in which to support large numbers of fish for research.  In no place is this trend more apparent than at Boston Children’s Hospital, which is home to one of the largest and most active zebrafish programs in the world.  The history of aquaculture infrastructure at BCH will be presented, with a primary focus on the two most recent improvement projects that together account for over 6 thousand square feet of space and over 10 thousand tank units.