ZEBRAFISH HUSBANDRY PROTOCOLS

The Zebrafish Husbandry Association (ZHA) provides these protocols only as reference material for its members and does not endorse or guarantee the accuracy, effectiveness, or results of their application.  ZHA is not liable for any unforeseen consequences associated with the use of these protocols. Protocols that describe the same procedure may vary based on the facility of origin. 

Microinjection
Microinjection - provided by Marc Tye, University of Minnesota
Pulling  Needles - provided by Marc Tye, University of Minnesota
Making Microinjection Trays - provided by Marc Tye, University of Minnesota
Dechorionating with Pronase
Dechorionating with Pronase - provided by Dante D'India, Harvard Medical School
Shipping Fish
Adults - provided by David Lains, ZIRC
Embryos  - provided by David Lains, ZIRC
In Vitro Fertilization
In Vitro Fertilization - provided by Dan Castranova, NIH
Freezing Sperm - provided by Dan Castranova, NIH
Euthanasia
Euthanasia - provided by Marc Tye, University of Minnesota
Solutions
Tricaine  - provided by Marc Tye, University of Minnesota
Tricaine - provided by Dan Castranova, NIH
Embryo Water  - provided by by Marc Tye, University of Minnesota
Embryo Water (Methylene Blue Water) - provided by Dan Castranova, NIH
Hanks Buffered Saline - provided by Dan Castranova, NIH
Bleaching Embryos
Bleaching Embryos - provided by Jim Burris, Duke University
Bleaching Embryos - provided by Dante D'India, Harvard
Bleaching Embryos - provided by Susan Farmer, UAB
Feeding
Feeding - provided by Jim Burris, Duke University
Rotifer Culture - provided by Marc Tye, University of Minnesota
Disinfecting Artemia - provided by Susan Farmer, UAB
Embryo Care
Embryo Care  - provided by Jim Burris, Duke University

If you would like to share your protocols please email them as a .pdf to admin@zhaonline.org.