ZEBRAFISH HUSBANDRY PROTOCOLS
The Zebrafish Husbandry Association (ZHA) provides these protocols only as reference material for its members and does not endorse or guarantee the accuracy, effectiveness, or results of their application. ZHA is not liable for any unforeseen consequences associated with the use of these protocols. Protocols that describe the same procedure may vary based on the facility of origin.
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Microinjection
Microinjection - provided by Marc Tye, University of Minnesota Pulling Needles - provided by Marc Tye, University of Minnesota Making Microinjection Trays - provided by Marc Tye, University of Minnesota Dechorionating with Pronase
Dechorionating with Pronase - provided by Dante D'India, Harvard Medical School In Vitro Fertilization
In Vitro Fertilization - provided by Dan Castranova, NIH Freezing Sperm - provided by Dan Castranova, NIH |
Solutions
Tricaine - provided by Marc Tye, University of Minnesota Tricaine - provided by Dan Castranova, NIH Embryo Water - provided by by Marc Tye, University of Minnesota Embryo Water (Methylene Blue Water) - provided by Dan Castranova, NIH Hanks Buffered Saline - provided by Dan Castranova, NIH Bleaching Embryos
Bleaching Embryos - provided by Jim Burris, Duke University Bleaching Embryos - provided by Dante D'India, Harvard Bleaching Embryos - provided by Susan Farmer, UAB Feeding
Feeding - provided by Jim Burris, Duke University Rotifer Culture - provided by Marc Tye, University of Minnesota Disinfecting Artemia - provided by Susan Farmer, UAB |
If you would like to share your protocols please email them as a .pdf to admin@zhaonline.org.
Protocols
Embryo Care
Euthanasia
Food
In Vitro Fertlization
In vitro fertilization- Castranova
Microinjection
Making microinjection trays- Tye
Room Logs
Coming soon!
Shipping Fish
Solutions
Embryo water (methylene blue)- Castranova